Assay

Functional Complement: Classical and Alternative Pathway Tests (CH100, AP100)

Key Words

Functional Complement Classical and Alternative Pathway Tests, CH100, AP100

Specimen Collection

Tests must be discussed with senior lab staff in advance as arrangements are required for rapid plasma and serum separation and freezing.

Turnaround time

28 days

This test is referred to another centre:                                                                                                                             Department of Clinical Chemistry and Immunology                                                                                                                   Level 4
Addenbrookes Hospital NHS Trust
Hills Road
Cambridge CB2 2QQ
Tel: 01223 217215
Fax: 01223 217794

Test indications

Two tests are performed to assess the function of both the classical and alternate complement pathways (CH100 and AP100).  These are Indicated for diagnosis of Complement deficiency and investigation of recurrent Neisserial infections. 

Interferences

Sample must be separated and frozen within 60 mins to prevent falsely low function results.                       

EDTA plasma samples should not be used as the chelation of calcium ions render some of the Complement components to be inactive.

Reference Range

CH100= 69-129%  

AP100  =    30-113%

Analytical error

Contact referral centre

Reference change value

N/A